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human pancreatic cancer cell lines bxpc3  (ATCC)


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    ATCC human pancreatic cancer cell lines bxpc3
    Human Pancreatic Cancer Cell Lines Bxpc3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pancreatic+cancer+cell+line+bxpc3/BxPC-3/pm42231052-160-0-12
    Average 99 stars, based on 4829 article reviews
    human pancreatic cancer cell lines bxpc3 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: STIP1 polypeptides and uses thereof
    Article Snippet: .. Preparation of Cell Culture Human ovarian cancer cell lines TOV112D, TOV21G, SKOV3, MDAH2774, ES2, MDAH2774, BG1; human endometrial cancer cell line RL95-2, human pancreatic cancer cell line BxPC3, breast cancer cell line (MCF7) and colon cancer cell line (HT29) were obtained from ATCC (Rockville, Md., USA). ..

    Article Title: Small extracellular vesicles promote stiffness-mediated metastasis
    Article Snippet: .. Human breast cancer cell lines MDA-MB-231, SUM149, and MDA-MB-468, human pancreatic cancer cell line BxPC3 (all from ATCC), and IMR90 human lung fibroblasts (gift from Daniele Gilkes, Johns Hopkins University) were cultured in 013-CV DMEM (Corning) containing 10% fetal bovine serum (Corning) and 1% penicillin-streptomycin (Gibco). .. After a 24 h incubation at 37°C, cells were washed with Dulbecco’s phosphate buffered saline (DPBS, Corning) and changed to 013-CV DMEM containing 10% exosome-depleted fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco).

    Article Title: STIP1 polypeptides and uses thereof
    Article Snippet: .. Preparation of Cell Culture Human ovarian cancer cell lines TOV112D, TOV21G, SKOV3, MDAH2774, ES2, MDAH2774,BG1; human endometrial cancer cell line RL95-2, human pancreatic cancer cell line BxPC3, breast cancer cell line (MCF7) and colon cancer cell line (HT29) were obtained from ATCC (Rockville, Md., USA). ..

    Article Title: Discovery of novel sitolactone derivative leading to PANoptosis and differentiation of acute myeloid leukemia cells.
    Article Snippet: Acute Myeloid Leukemia (AML) is a devastating hematologic malignancy.. Chemotherapy remains the primary treatment, offering rapid disease control and potential complete remission.. However, more than half of the patients develop resistance and relapse, significantly reducing patient survival.

    Article Title: Targeted glycomics by selected reaction monitoring for highly sensitive glycan compositional analysis
    Article Snippet: .. After mixing the samples for 30 s and standing at room temperature for 5 min, 10 units of PNGase F were added, followed by incubation at 37°C for 24 h. The cells used in this study were human pancreatic cancer cell line BxPC3 obtained from the American Type Culture Collection, ATCC (Manassas, VA), and cultured in RPMI medium (Invitrogen, Carlsbad, CA). .. Foetal bovine serum (Roche, Mississauga, ON) at 10% of the final volume was added to the medium beforehand.

    Multiple Displacement Amplification:

    Article Title: Small extracellular vesicles promote stiffness-mediated metastasis
    Article Snippet: .. Human breast cancer cell lines MDA-MB-231, SUM149, and MDA-MB-468, human pancreatic cancer cell line BxPC3 (all from ATCC), and IMR90 human lung fibroblasts (gift from Daniele Gilkes, Johns Hopkins University) were cultured in 013-CV DMEM (Corning) containing 10% fetal bovine serum (Corning) and 1% penicillin-streptomycin (Gibco). .. After a 24 h incubation at 37°C, cells were washed with Dulbecco’s phosphate buffered saline (DPBS, Corning) and changed to 013-CV DMEM containing 10% exosome-depleted fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco).

    Article Title: Kremen1 dependence receptor induces SEC24C and ATG9A-dependent cell death
    Article Snippet: The pcDNA3 APEX2-NES (addgene #49,386) was used to fuse the APEX2 enzyme at the C-terminus of Kremen1 in the Psbtet plasmid using infusion cloning. .. The human breast cancer cell line MDA-MB231, human pancreatic cancer cell line BxPC3 and human embryonic kidney HEK293T cells were obtained from ATCC (UK) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. .. For the establishment of stable cell lines MDA-MB231, BxPC3 and HEK293T were transfected with psbtetGFP-Kremen1 or psbtetRFP-Kremen1, (1.6 μg plasmid) and 0.4 μg SB100x transposase and Fugen (Promega) in a ratio of total DNA: transfectant of 1:3 and then selected with puromycin (Gibco Life Technologies) or zeocin (Invitrogen) for 2 to 4 weeks.

    Article Title: Kremen1 dependence receptor induces SEC24C and ATG9A-dependent cell death.
    Article Snippet: The pcDNA3 APEX2-NES (addgene #49386) was used to fuse the APEX2 enzyme at the C-terminus of Kremen1 in the Psbtet plasmid using infusion cloning. .. The human breast cancer cell line MDA-MB231, human pancreatic cancer cell line BxPC3 and human embryonic kidney HEK293T cells were obtained from ATCC (UK) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. .. For the establishment of stable cell lines MDA-MB231, BxPC3 and HEK293T were transfected with psbtetGFP-Kremen1 or psbtetRFP-Kremen1, (1.6 μg plasmid) and 0.4 μg SB100x transposase and Fugen (Promega) in a ratio of total DNA: transfectant of 1:3 and then selected with puromycin (Gibco Life Technologies) or zeocin (Invitrogen) for 2 to 4 weeks.

    Modification:

    Article Title: Kremen1 dependence receptor induces SEC24C and ATG9A-dependent cell death
    Article Snippet: The pcDNA3 APEX2-NES (addgene #49,386) was used to fuse the APEX2 enzyme at the C-terminus of Kremen1 in the Psbtet plasmid using infusion cloning. .. The human breast cancer cell line MDA-MB231, human pancreatic cancer cell line BxPC3 and human embryonic kidney HEK293T cells were obtained from ATCC (UK) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. .. For the establishment of stable cell lines MDA-MB231, BxPC3 and HEK293T were transfected with psbtetGFP-Kremen1 or psbtetRFP-Kremen1, (1.6 μg plasmid) and 0.4 μg SB100x transposase and Fugen (Promega) in a ratio of total DNA: transfectant of 1:3 and then selected with puromycin (Gibco Life Technologies) or zeocin (Invitrogen) for 2 to 4 weeks.

    Article Title: Kremen1 dependence receptor induces SEC24C and ATG9A-dependent cell death.
    Article Snippet: The pcDNA3 APEX2-NES (addgene #49386) was used to fuse the APEX2 enzyme at the C-terminus of Kremen1 in the Psbtet plasmid using infusion cloning. .. The human breast cancer cell line MDA-MB231, human pancreatic cancer cell line BxPC3 and human embryonic kidney HEK293T cells were obtained from ATCC (UK) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. .. For the establishment of stable cell lines MDA-MB231, BxPC3 and HEK293T were transfected with psbtetGFP-Kremen1 or psbtetRFP-Kremen1, (1.6 μg plasmid) and 0.4 μg SB100x transposase and Fugen (Promega) in a ratio of total DNA: transfectant of 1:3 and then selected with puromycin (Gibco Life Technologies) or zeocin (Invitrogen) for 2 to 4 weeks.

    Incubation:

    Article Title: Targeted glycomics by selected reaction monitoring for highly sensitive glycan compositional analysis
    Article Snippet: .. After mixing the samples for 30 s and standing at room temperature for 5 min, 10 units of PNGase F were added, followed by incubation at 37°C for 24 h. The cells used in this study were human pancreatic cancer cell line BxPC3 obtained from the American Type Culture Collection, ATCC (Manassas, VA), and cultured in RPMI medium (Invitrogen, Carlsbad, CA). .. Foetal bovine serum (Roche, Mississauga, ON) at 10% of the final volume was added to the medium beforehand.



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    human pancreatic cancer cell lines bxpc3  (ATCC)
    99
    ATCC human pancreatic cancer cell lines bxpc3
    Human Pancreatic Cancer Cell Lines Bxpc3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pancreatic+cancer+cell+line+bxpc3/BxPC-3/pm42231052-160-0-12
    Average 99 stars, based on 1 article reviews
    human pancreatic cancer cell lines bxpc3 - by Bioz Stars, 2026-09
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    human pancreatic cancer cell line bxpc3  (ATCC)
    99
    ATCC human pancreatic cancer cell line bxpc3
    Human Pancreatic Cancer Cell Line Bxpc3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pancreatic+cancer+cell+line+bxpc3/BxPC-3/pm41807954-184-7-22
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    human pancreatic cancer cell line bxpc3 - by Bioz Stars, 2026-09
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    bxpc3 human pancreatic cancer cell lines  (ATCC)
    99
    ATCC bxpc3 human pancreatic cancer cell lines
    Fig. 2 Effect of Ps conditioned media on PDAC growth. (A) Growth curve of HuPa4 tumors transplanted s.c and treated with HPs conditioned medium or control medium (mean ± SEM; n = 4 for each group, two-way ANOVA with Bonferroni’s multiple comparison test, *p < 0.005,**p < 0.0005, ***p < 0.0001). Ki67 immunostaining (B) and quantification of tumor cell proliferation (C) at euthanasia (400X, scale bar 100 μm) (mean ± SEM). Proliferation of MIAPaCa2 (D), <t>BxPC3</t> (E), and FC1199 cells (F) in response to respectively 7 and 5 human and murine Ps CM, (72 h of treatment). Data are percentages of control from at least two different experiments (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, ***p < 0.001 ****p < 0.0001). (G) Proliferation of FC1199 cells in response to murine Ps CM, containing or not Langerhans islets. Data are percentages of control from at least five different Ps preparations (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, **p < 0.01, *p < 0.05)
    Bxpc3 Human Pancreatic Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pancreatic+cancer+cell+line+bxpc3/BxPC-3/pm37559126-37-3-20
    Average 99 stars, based on 1 article reviews
    bxpc3 human pancreatic cancer cell lines - by Bioz Stars, 2026-09
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    Fig. 2 Effect of Ps conditioned media on PDAC growth. (A) Growth curve of HuPa4 tumors transplanted s.c and treated with HPs conditioned medium or control medium (mean ± SEM; n = 4 for each group, two-way ANOVA with Bonferroni’s multiple comparison test, *p < 0.005,**p < 0.0005, ***p < 0.0001). Ki67 immunostaining (B) and quantification of tumor cell proliferation (C) at euthanasia (400X, scale bar 100 μm) (mean ± SEM). Proliferation of MIAPaCa2 (D), BxPC3 (E), and FC1199 cells (F) in response to respectively 7 and 5 human and murine Ps CM, (72 h of treatment). Data are percentages of control from at least two different experiments (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, ***p < 0.001 ****p < 0.0001). (G) Proliferation of FC1199 cells in response to murine Ps CM, containing or not Langerhans islets. Data are percentages of control from at least five different Ps preparations (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, **p < 0.01, *p < 0.05)

    Journal: Journal of experimental & clinical cancer research : CR

    Article Title: Fibronectin fragments generated by pancreatic trypsin act as endogenous inhibitors of pancreatic tumor growth.

    doi: 10.1186/s13046-023-02778-y

    Figure Lengend Snippet: Fig. 2 Effect of Ps conditioned media on PDAC growth. (A) Growth curve of HuPa4 tumors transplanted s.c and treated with HPs conditioned medium or control medium (mean ± SEM; n = 4 for each group, two-way ANOVA with Bonferroni’s multiple comparison test, *p < 0.005,**p < 0.0005, ***p < 0.0001). Ki67 immunostaining (B) and quantification of tumor cell proliferation (C) at euthanasia (400X, scale bar 100 μm) (mean ± SEM). Proliferation of MIAPaCa2 (D), BxPC3 (E), and FC1199 cells (F) in response to respectively 7 and 5 human and murine Ps CM, (72 h of treatment). Data are percentages of control from at least two different experiments (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, ***p < 0.001 ****p < 0.0001). (G) Proliferation of FC1199 cells in response to murine Ps CM, containing or not Langerhans islets. Data are percentages of control from at least five different Ps preparations (mean ± SEM; one-way ANOVA with Dunnett’s multiple comparison test, **p < 0.01, *p < 0.05)

    Article Snippet: The MIAPaCa2 and BxPC3 human pancreatic cancer cell lines were obtained, respectively, from Istituto Zooprofilattico Sperimentale (Brescia, Italy) and from American Type Culture Collection (ATCC) and authenticated using the AmpFlSTR® Identifiler® PCR Amplification Kit (Applied Biosystems, Merk, Milano, Italy).

    Techniques: Control, Comparison, Immunostaining